immunocytochemical method meaning in English
免疫细胞化学法
Examples
- Electron microscopy and immunocytochemical method were used to evaluate the cultivated cells
结果：培养的周细胞贴壁生长，胞体肥大，呈不规则状、有较多突起。 - An immunocytochemical method for identification of bursaphelenchus xylophilus antigen on the surface of pinewood section
疫木切面上松材线虫抗原的免疫学检测方法 - Mdr1 express product p - glycoprotein was detected by immunocytochemical method and flowcytometry . the cytotoxicity and multidrug resistance reversion effect of tea polyphenol was examined by mtt assay in mcf - 7 and mcf - 7 adr carcinoma cell lines , and compared with pgp inhibitor quinidine . the pgp expression of mcf - 7 adr was strongly positive , the positive rate was 15 % ; the pgp expression of mcf - 7 was negative , the positive rate was 1 . 8 % . ic50 of tea polyphenol to mcf - 7 and mcf - 7 adr is 115 . 2g ml and 207 . 6g ml respectively . ic50 of quinidine to mcf - 7 and mcf - 7 adr is 129 . 8mol l 42 . 1g ml and 94 . 1mol l 30 . 5g ml respectively . tea polyphenol and quinidine changed little toxicity of adriamycin to mcf - 7 , but tea polyphenol and quinidine improved the sensitivity of mcf - 7adr to adriamycin significantly . immunocytochemistry and flow cytometry can detect p - glycoprotein expression level qualitatively and quantitatively . tea polyphenol is not only an anti - tumor agent , but also a multidrug resistant modulator similar as quinidine . tea polyphenol is advantageous for its little toxicity in tumor treatment
用免疫组化法和流式细胞仪对肿瘤细胞系mcf - 7和mcf - 7 adr的p -糖蛋白表达水平进行定性定量研究。用噻唑蓝比色法mtt研究茶多酚的细胞毒性及其对耐药性的逆转作用，并与pgp抑制剂奎尼定进行了比较。免疫组化法检测p -糖蛋白表达水平， mcf - 7 adr呈强阳性，而mcf - 7呈阴性流式细胞仪定量检测结果mcf - 7 adr细胞系细胞阳性率为15 % ， mcf - 7细胞系细胞阳性率为1 . 8 % 。